Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3274548..3275345 Replicon chromosome
Accession NZ_CP056747
Organism Enterobacter hormaechei strain RHBSTW-00217

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag HV129_RS15625 Protein ID WP_032670168.1
Coordinates 3274548..3275069 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag HV129_RS15630 Protein ID WP_001303459.1
Coordinates 3275076..3275345 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV129_RS15595 3270147..3270836 + 690 WP_047052685.1 pyrimidine utilization protein B -
HV129_RS15600 3270848..3271234 + 387 WP_032670161.1 pyrimidine utilization protein C -
HV129_RS15605 3271242..3272041 + 800 Protein_3046 pyrimidine utilization protein D -
HV129_RS15610 3272051..3272641 + 591 WP_047052687.1 malonic semialdehyde reductase -
HV129_RS15615 3272654..3273145 + 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
HV129_RS15620 3273167..3274489 + 1323 WP_048982825.1 pyrimidine utilization transport protein G -
HV129_RS15625 3274548..3275069 - 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
HV129_RS15630 3275076..3275345 - 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
HV129_RS15635 3275420..3276325 - 906 WP_047051684.1 DMT family transporter -
HV129_RS15640 3276442..3276612 - 171 WP_001273664.1 general stress protein -
HV129_RS15645 3277004..3277600 + 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
HV129_RS15650 3277619..3277846 + 228 WP_006809324.1 YccJ family protein -
HV129_RS15655 3277879..3279120 - 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
HV129_RS15660 3279446..3279805 - 360 WP_040117469.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T164115 WP_032670168.1 NZ_CP056747:c3275069-3274548 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T164115 NZ_CP072793:350072-350329 [Thiothrix unzii]
ATGAACGCCATTTCCTACACCGCTGTGCGAGCGGATCTCGCCAAAACGATGGATCGGGTCTGCGAAGACCATGAACCCGT
CATTATTACCCGCAACCGCGATCAAGCCGTGGTGATGATTTCGCTGGAAGATTACAAAGCGATGGAAGAAACCGCGTATT
TATTGCGTAGTCCAGCCAATGCCAGCCGCTTGCTGGAGTCCATTGCGGAATTGGAGCGTGGTCAAGGGCAAGTGCGTGAG
TTGATCGAATGCGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT164115 WP_001303459.1 NZ_CP056747:c3275345-3275076 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT164115 NZ_CP072793:350320-350577 [Thiothrix unzii]
ATGCGAATAATCTTTTCATCCAAAGCGTGGGAGGATTACGGGCATTGGCAACAAGCCGATAAGAAAATTCTCAAGCGTAT
CAATGATCTGATCAAAGCGATTTCGCGTGAACCGTTCGAGGGTATCGGCAAGCCCGAACCATTGCGGCACGGGCTTTCTG
GCTATTGGTCACGCCGTATCAATGATGAACATCGGCTGGTCTACAAAGTGGAAGGGGAAGACTTGCTGATAGCGATGTGC
CGCTATCACTACCCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References