Detailed information of TA system TA164103

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1012753..1013432
• Replicon: chromosome
• Accession: NZ_CP056747
• Organism: Enterobacter hormaechei strain RHBSTW-00217

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A411GL63
• Locus tag: HV129_RS04820
• Protein ID: WP_016243829.1
• Coordinates: 1013091..1013432 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B2XJW9
• Locus tag: HV129_RS04815
• Protein ID: WP_016243830.1
• Coordinates: 1012753..1013070 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV129_RS04770	1008188..1009009	+	822	WP_008324532.1	DUF945 domain-containing protein	-
HV129_RS04775	1009226..1009927	+	702	WP_047749107.1	WYL domain-containing protein	-
HV129_RS04780	1009964..1010200	+	237	WP_001115854.1	hypothetical protein	-
HV129_RS04785	1010200..1010643	+	444	WP_016243834.1	hypothetical protein	-
HV129_RS04790	1010667..1011134	+	468	WP_016239439.1	hypothetical protein	-
HV129_RS04795	1011211..1011450	+	240	WP_016243833.1	DUF905 domain-containing protein	-
HV129_RS04800	1011552..1012010	+	459	WP_016243832.1	antirestriction protein	-
HV129_RS04805	1012026..1012502	+	477	WP_016243831.1	RadC family protein	-
HV129_RS04810	1012511..1012732	+	222	WP_007777714.1	DUF987 domain-containing protein	-
HV129_RS04815	1012753..1013070	+	318	WP_016243830.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV129_RS04820	1013091..1013432	+	342	WP_016243829.1	TA system toxin CbtA family protein	Toxin
HV129_RS04830	1013794..1014300	+	507	WP_032649590.1	G/U mismatch-specific DNA glycosylase	-
HV129_RS04835	1014390..1016234	-	1845	WP_006812010.1	RNA polymerase sigma factor RpoD	-
HV129_RS04840	1016388..1018133	-	1746	WP_003862574.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	998609..1013432	14823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13020.04 Da
• Isoelectric Point: 9.9961

>T164103 WP_016243829.1 NZ_CP056747:1013091-1013432 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T164103 NZ_CP072790:c3104667-3104311 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12041.72 Da
• Isoelectric Point: 7.7518

>AT164103 WP_016243830.1 NZ_CP056747:1012753-1013070 [Enterobacter hormaechei]
MTHITWGLQRNITPRLGARLVQEGNRMHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEMNPRNAHCVTLFH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT164103 NZ_CP072790:c3104881-3104651 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A411GL63

Antitoxin

• AlphaFold DB: A0A8B2XJW9