Detailed information of TA system TA164102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 322630..323246
• Replicon: chromosome
• Accession: NZ_CP056747
• Organism: Enterobacter hormaechei strain RHBSTW-00217

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV129_RS01575
• Protein ID: WP_077266491.1
• Coordinates: 322875..323246 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HV129_RS01570
• Protein ID: WP_015569912.1
• Coordinates: 322630..322872 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV129_RS01540	317689..318489	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV129_RS01545	318608..319207	+	600	WP_032649898.1	glucose-1-phosphatase	-
HV129_RS01550	319201..320082	+	882	WP_003861949.1	virulence factor BrkB family protein	-
HV129_RS01555	320079..320516	+	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV129_RS01560	320561..321502	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HV129_RS01565	321511..322431	-	921	WP_047051644.1	alpha/beta hydrolase	-
HV129_RS01570	322630..322872	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV129_RS01575	322875..323246	+	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV129_RS01580	323286..324215	-	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
HV129_RS01585	324212..324847	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
HV129_RS01590	324844..325746	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T164102 WP_077266491.1 NZ_CP056747:322875-323246 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T164102 NZ_CP072790:c3065414-3065019 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT164102 WP_015569912.1 NZ_CP056747:322630-322872 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT164102 NZ_CP072790:c3064993-3064727 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3