Detailed information of TA system TA164097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4689565..4690181
• Replicon: chromosome
• Accession: NZ_CP056742
• Organism: Enterobacter hormaechei strain RHBSTW-00220

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV132_RS22650
• Protein ID: WP_077266491.1
• Coordinates: 4689565..4689936 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HV132_RS22655
• Protein ID: WP_015569912.1
• Coordinates: 4689939..4690181 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV132_RS22635	4687065..4687967	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
HV132_RS22640	4687964..4688599	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
HV132_RS22645	4688596..4689525	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
HV132_RS22650	4689565..4689936	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV132_RS22655	4689939..4690181	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV132_RS22660	4690380..4691300	+	921	WP_047051644.1	alpha/beta hydrolase	-
HV132_RS22665	4691309..4692250	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HV132_RS22670	4692295..4692732	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV132_RS22675	4692729..4693610	-	882	WP_003861949.1	virulence factor BrkB family protein	-
HV132_RS22680	4693604..4694203	-	600	WP_032649898.1	glucose-1-phosphatase	-
HV132_RS22685	4694322..4695122	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T164097 WP_077266491.1 NZ_CP056742:c4689936-4689565 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T164097 NZ_CP072790:2864257-2864634 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTACTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT164097 WP_015569912.1 NZ_CP056742:c4690181-4689939 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT164097 NZ_CP072790:2864003-2864260 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3