Detailed information of TA system TA164074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 25871..26514
• Replicon: plasmid pRHBSTW-00309_2
• Accession: NZ_CP056738
• Organism: Enterobacter roggenkampii strain RHBSTW-00309

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7D6UII7
• Locus tag: HV150_RS24775
• Protein ID: WP_032425563.1
• Coordinates: 25871..26287 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HV150_RS24780
• Protein ID: WP_001261276.1
• Coordinates: 26284..26514 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV150_RS24755	23050..23619	-	570	WP_023332907.1	small heat shock protein sHSP20	-
HV150_RS24760	23654..23935	-	282	WP_023332908.1	helix-turn-helix domain-containing protein	-
HV150_RS24765	24129..24392	-	264	WP_001295708.1	hypothetical protein	-
HV150_RS24770	24552..25520	+	969	WP_016151349.1	IS5 family transposase	-
HV150_RS24775	25871..26287	-	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV150_RS24780	26284..26514	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV150_RS24785	27070..27492	-	423	WP_181544268.1	hypothetical protein	-
HV150_RS24790	27498..28202	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HV150_RS24795	28446..28736	+	291	WP_001247892.1	nucleotidyltransferase	-
HV150_RS24800	28733..29134	+	402	WP_001293886.1	DUF86 domain-containing protein	-
HV150_RS24805	29124..29480	+	357	WP_000215515.1	cupin domain-containing protein	-
HV150_RS24810	29735..30061	+	327	WP_003100858.1	hypothetical protein	-
HV150_RS24815	30058..30558	+	501	WP_003100856.1	hypothetical protein	-
HV150_RS24820	30555..30926	+	372	WP_003100853.1	hypothetical protein	-
HV150_RS24825	30920..31477	+	558	WP_003100847.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..149353	149353
	-	inside	IScluster/Tn	-	-	24552..36433	11881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.56 Da
• Isoelectric Point: 8.5403

>T164074 WP_032425563.1 NZ_CP056738:c26287-25871 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T164074 NZ_CP072790:c1237390-1237079 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT164074 WP_001261276.1 NZ_CP056738:c26514-26284 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT164074 NZ_CP072790:c1237710-1237390 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Toxin

• AlphaFold DB: A0A7D6UII7

Antitoxin

• AlphaFold DB: A0A387K023