Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4316663..4317342 Replicon chromosome
Accession NZ_CP056737
Organism Enterobacter roggenkampii strain RHBSTW-00309

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7D6Z078
Locus tag HV150_RS20820 Protein ID WP_048215514.1
Coordinates 4316663..4317004 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0F6RHY7
Locus tag HV150_RS20825 Protein ID WP_046495841.1
Coordinates 4317025..4317342 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV150_RS20775 4311675..4311902 - 228 WP_063922910.1 TraR/DksA family transcriptional regulator -
HV150_RS20780 4311902..4312135 - 234 WP_001744223.1 DUF2732 domain-containing protein -
HV150_RS20785 4312203..4312544 - 342 WP_048971665.1 DUF5347 domain-containing protein -
HV150_RS20790 4312508..4312708 - 201 WP_032442472.1 DUF2724 domain-containing protein -
HV150_RS20795 4312716..4313225 - 510 WP_181544009.1 phage regulatory CII family protein -
HV150_RS20800 4313260..4313496 - 237 WP_015386350.1 hypothetical protein -
HV150_RS20805 4313598..4314212 + 615 WP_015386349.1 phage repressor protein CI -
HV150_RS20810 4314439..4315452 + 1014 WP_015386347.1 tyrosine-type recombinase/integrase -
HV150_RS20815 4315714..4316547 - 834 WP_046495848.1 DUF4942 domain-containing protein -
HV150_RS20820 4316663..4317004 - 342 WP_048215514.1 TA system toxin CbtA family protein Toxin
HV150_RS20825 4317025..4317342 - 318 WP_046495841.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV150_RS20830 4317361..4317582 - 222 WP_000691995.1 DUF987 domain-containing protein -
HV150_RS20835 4317591..4318067 - 477 WP_046495838.1 RadC family protein -
HV150_RS20840 4318083..4318541 - 459 WP_046495834.1 antirestriction protein -
HV150_RS20845 4318620..4318856 - 237 WP_046495830.1 DUF905 domain-containing protein -
HV150_RS20850 4318934..4319344 - 411 WP_046495825.1 hypothetical protein -
HV150_RS20855 4319433..4320047 - 615 WP_046495819.1 hypothetical protein -
HV150_RS20860 4320044..4320748 - 705 WP_046495813.1 WYL domain-containing protein -
HV150_RS20865 4320950..4321783 - 834 WP_048215513.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4277024..4321783 44759


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(2-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13014.97 Da        Isoelectric Point: 10.4182

>T164073 WP_048215514.1 NZ_CP056737:c4317004-4316663 [Enterobacter roggenkampii]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T164073 NZ_CP072790:1174136-1175017 [Mycobacterium tuberculosis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11869.49 Da        Isoelectric Point: 6.6245

>AT164073 WP_046495841.1 NZ_CP056737:c4317342-4317025 [Enterobacter roggenkampii]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT164073 NZ_CP072790:1173516-1174139 [Mycobacterium tuberculosis]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6Z078

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RHY7

References