Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 581713..582289 Replicon chromosome
Accession NZ_CP056737
Organism Enterobacter roggenkampii strain RHBSTW-00309

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag HV150_RS02770 Protein ID WP_025912099.1
Coordinates 581713..582000 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7D6YIG8
Locus tag HV150_RS02775 Protein ID WP_025912101.1
Coordinates 581987..582289 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV150_RS02740 576726..576842 + 117 Protein_529 amino acid-binding protein -
HV150_RS02745 576835..577530 + 696 WP_023293362.1 winged helix-turn-helix domain-containing protein -
HV150_RS02750 577676..578146 + 471 WP_008501325.1 MarR family transcriptional regulator -
HV150_RS02755 578143..579210 + 1068 WP_100245850.1 HlyD family secretion protein -
HV150_RS02760 579200..580276 + 1077 WP_045335580.1 DUF2955 domain-containing protein -
HV150_RS02765 580273..581544 - 1272 WP_045353153.1 DUF445 domain-containing protein -
HV150_RS02770 581713..582000 + 288 WP_025912099.1 BrnT family toxin Toxin
HV150_RS02775 581987..582289 + 303 WP_025912101.1 BrnA antitoxin family protein Antitoxin
HV150_RS02780 582320..582958 - 639 WP_106106435.1 LysE family translocator -
HV150_RS02785 583006..583749 - 744 WP_106106434.1 AraC family transcriptional regulator -
HV150_RS02790 583901..585271 + 1371 WP_106106433.1 NAD-dependent succinate-semialdehyde dehydrogenase -
HV150_RS02795 585317..585637 - 321 WP_008501314.1 hypothetical protein -
HV150_RS02800 585637..586182 - 546 WP_008501313.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T164058 WP_025912099.1 NZ_CP056737:581713-582000 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T164058 NZ_CP072790:c449319-448726 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11413.02 Da        Isoelectric Point: 10.0592

>AT164058 WP_025912101.1 NZ_CP056737:581987-582289 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARREAELKALAEKPEAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT164058 NZ_CP072790:c449737-449348 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A7D6YIG8

References