Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19147..19790 Replicon plasmid pRHBSTW-00316_4
Accession NZ_CP056730
Organism Enterobacter hormaechei strain RHBSTW-00316

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag HV155_RS24125 Protein ID WP_000754567.1
Coordinates 19374..19790 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag HV155_RS24120 Protein ID WP_023293777.1
Coordinates 19147..19377 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV155_RS24090 14148..14414 + 267 Protein_12 hypothetical protein -
HV155_RS24095 14808..15674 - 867 WP_023302476.1 replication initiation protein -
HV155_RS24100 16209..16322 + 114 WP_032427600.1 hypothetical protein -
HV155_RS24105 16451..16708 + 258 WP_000764642.1 hypothetical protein -
HV155_RS24110 16766..17554 - 789 WP_022631000.1 site-specific integrase -
HV155_RS24115 17574..18245 - 672 WP_024140048.1 hypothetical protein -
HV155_RS24120 19147..19377 + 231 WP_023293777.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV155_RS24125 19374..19790 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
HV155_RS24130 20141..21109 - 969 WP_016151349.1 IS5 family transposase -
HV155_RS24135 21404..22429 - 1026 WP_001101446.1 IS110 family transposase -
HV155_RS24140 22461..22628 - 168 WP_074187713.1 transposase -
HV155_RS24145 22643..22906 + 264 WP_063842267.1 hypothetical protein -
HV155_RS24150 23100..23381 + 282 WP_023332908.1 helix-turn-helix domain-containing protein -
HV155_RS24155 23416..23985 + 570 WP_023332907.1 small heat shock protein sHSP20 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48470 48470


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T164056 WP_000754567.1 NZ_CP056730:19374-19790 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T164056 NZ_CP072790:367944-368246 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT164056 WP_023293777.1 NZ_CP056730:19147-19377 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT164056 NZ_CP072790:368294-368515 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References