Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5036..5561 Replicon plasmid pRHBSTW-00316_3
Accession NZ_CP056729
Organism Enterobacter hormaechei strain RHBSTW-00316

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag HV155_RS23650 Protein ID WP_006788214.1
Coordinates 5036..5341 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV155_RS23655 Protein ID WP_006788213.1
Coordinates 5343..5561 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV155_RS23630 1487..2113 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
HV155_RS23635 2233..2412 + 180 WP_006788217.1 hypothetical protein -
HV155_RS23640 2836..3630 - 795 WP_016240356.1 site-specific integrase -
HV155_RS23645 3866..4351 - 486 Protein_4 hypothetical protein -
HV155_RS23650 5036..5341 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
HV155_RS23655 5343..5561 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV155_RS23660 5731..5976 - 246 Protein_7 hypothetical protein -
HV155_RS23665 6500..7957 - 1458 WP_057072997.1 amidohydrolase -
HV155_RS23670 8067..8933 - 867 WP_057072971.1 transporter substrate-binding domain-containing protein -
HV155_RS23675 9014..9778 - 765 WP_057072961.1 ATP-binding cassette domain-containing protein -
HV155_RS23680 9775..10503 - 729 WP_057072996.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkF / mrkD / mrkC / mrkB / mrkA 1..87112 87112


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T164055 WP_006788214.1 NZ_CP056729:c5341-5036 [Enterobacter hormaechei]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T164055 NZ_CP072790:367944-368246 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT164055 WP_006788213.1 NZ_CP056729:c5561-5343 [Enterobacter hormaechei]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT164055 NZ_CP072790:367720-367947 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References