Detailed information of TA system TA164043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2272907..2273646
• Replicon: chromosome
• Accession: NZ_CP056727
• Organism: Enterobacter hormaechei strain RHBSTW-00316

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV155_RS10850
• Protein ID: WP_063139083.1
• Coordinates: 2272907..2273392 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: HV155_RS10855
• Protein ID: WP_003857131.1
• Coordinates: 2273380..2273646 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV155_RS10825	2268725..2269483	-	759	WP_032624253.1	trans-aconitate 2-methyltransferase	-
HV155_RS10830	2269568..2270152	-	585	WP_022648128.1	NUDIX hydrolase	-
HV155_RS10835	2270239..2270997	+	759	WP_022648129.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV155_RS10840	2271102..2272394	+	1293	WP_045622665.1	glycoside hydrolase	-
HV155_RS10845	2272681..2272881	+	201	Protein_2111	hypothetical protein	-
HV155_RS10850	2272907..2273392	-	486	WP_063139083.1	GNAT family N-acetyltransferase	Toxin
HV155_RS10855	2273380..2273646	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
HV155_RS10860	2273710..2274639	-	930	WP_063139082.1	LysR family transcriptional regulator	-
HV155_RS10865	2274769..2276157	+	1389	WP_045622667.1	MFS transporter	-
HV155_RS10870	2276136..2276693	-	558	WP_045622668.1	OmpH family outer membrane protein	-
HV155_RS10875	2276814..2277950	-	1137	WP_045622670.1	type 1 fimbrial protein	-
HV155_RS10880	2277974..2278498	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2258998..2273646	14648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17544.22 Da
• Isoelectric Point: 9.6275

>T164043 WP_063139083.1 NZ_CP056727:c2273392-2272907 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T164043 NZ_CP072787:c2805883-2805781 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT164043 WP_003857131.1 NZ_CP056727:c2273646-2273380 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT164043 NZ_CP072787:2805743-2806011 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCTAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9