Detailed information of TA system TA164040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 34121..34758
• Replicon: plasmid pRHBSTW-00333_3
• Accession: NZ_CP056721
• Organism: Enterobacter hormaechei strain RHBSTW-00333

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: HV163_RS25105
• Protein ID: WP_032662169.1
• Coordinates: 34121..34531 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: HV163_RS25110
• Protein ID: WP_032662167.1
• Coordinates: 34528..34758 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV163_RS25085	29486..29836	+	351	WP_032662182.1	DUF305 domain-containing protein	-
HV163_RS25090	30022..30930	-	909	WP_032662179.1	HNH endonuclease	-
HV163_RS25095	31478..32500	-	1023	WP_040119796.1	helicase UvrD	-
HV163_RS25100	32485..34047	-	1563	WP_181561688.1	AAA family ATPase	-
HV163_RS25105	34121..34531	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
HV163_RS25110	34528..34758	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV163_RS25115	35104..38229	+	3126	WP_040119768.1	hypothetical protein	-
HV163_RS25120	38516..38806	+	291	WP_040119769.1	hypothetical protein	-
HV163_RS25125	38796..39695	+	900	WP_040119770.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118204	118204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T164040 WP_032662169.1 NZ_CP056721:c34531-34121 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T164040 NZ_CP072787:c2230784-2230677 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT164040 WP_032662167.1 NZ_CP056721:c34758-34528 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT164040 NZ_CP072787:2230840-2230901 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTCT