Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3824378..3824998 Replicon chromosome
Accession NZ_CP056717
Organism Citrobacter sp. RHBSTW-00599

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV243_RS18755 Protein ID WP_002892050.1
Coordinates 3824780..3824998 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7L6U5G9
Locus tag HV243_RS18750 Protein ID WP_019076175.1
Coordinates 3824378..3824752 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV243_RS18740 3819525..3820718 + 1194 WP_053389373.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV243_RS18745 3820741..3823890 + 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV243_RS18750 3824378..3824752 + 375 WP_019076175.1 Hha toxicity modulator TomB Antitoxin
HV243_RS18755 3824780..3824998 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV243_RS18760 3825179..3825730 + 552 WP_016152072.1 maltose O-acetyltransferase -
HV243_RS18765 3825847..3826317 + 471 WP_016152071.1 YlaC family protein -
HV243_RS18770 3826396..3826536 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV243_RS18775 3826538..3826798 - 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV243_RS18780 3826987..3828540 + 1554 WP_053388979.1 EAL domain-containing protein -
HV243_RS18785 3828592..3828945 - 354 WP_003831033.1 DUF1428 family protein -
HV243_RS18790 3829010..3829639 - 630 WP_049270150.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T164021 WP_002892050.1 NZ_CP056717:3824780-3824998 [Citrobacter sp. RHBSTW-00599]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T164021 NZ_CP072786:c2458275-2458006 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14413.20 Da        Isoelectric Point: 5.5653

>AT164021 WP_019076175.1 NZ_CP056717:3824378-3824752 [Citrobacter sp. RHBSTW-00599]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT164021 NZ_CP072786:c2458006-2457722 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U5G9

References