Detailed information of TA system TA164012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2550866..2551502
• Replicon: chromosome
• Accession: NZ_CP056717
• Organism: Citrobacter sp. RHBSTW-00599

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV243_RS12465
• Protein ID: WP_049259794.1
• Coordinates: 2550866..2551054 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV243_RS12470
• Protein ID: WP_131382557.1
• Coordinates: 2551086..2551502 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV243_RS12445	2547644..2547874	-	231	WP_016153158.1	DUF2554 family protein	-
HV243_RS12450	2548064..2548189	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV243_RS12455	2548189..2549199	-	1011	WP_016153157.1	cytochrome d ubiquinol oxidase subunit II	-
HV243_RS12460	2549199..2550602	-	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
HV243_RS12465	2550866..2551054	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV243_RS12470	2551086..2551502	+	417	WP_131382557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV243_RS12475	2551595..2553004	+	1410	WP_019076828.1	PLP-dependent aminotransferase family protein	-
HV243_RS12480	2553334..2554479	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV243_RS12485	2554496..2555512	+	1017	WP_053389782.1	ABC transporter ATP-binding protein	-
HV243_RS12490	2555513..2556457	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T164012 WP_049259794.1 NZ_CP056717:2550866-2551054 [Citrobacter sp. RHBSTW-00599]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T164012 NZ_CP072786:470542-470715 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.33 Da
• Isoelectric Point: 4.7119

>AT164012 WP_131382557.1 NZ_CP056717:2551086-2551502 [Citrobacter sp. RHBSTW-00599]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT164012 NZ_CP072786:470218-470514 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9