Detailed information of TA system TA164011

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 935873..936527
• Replicon: chromosome
• Accession: NZ_CP056717
• Organism: Citrobacter sp. RHBSTW-00599

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV243_RS04510
• Protein ID: WP_016154348.1
• Coordinates: 936120..936527 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV243_RS04505
• Protein ID: WP_016154349.1
• Coordinates: 935873..936139 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV243_RS04480	931086..932519	-	1434	WP_053389494.1	6-phospho-beta-glucosidase BglA	-
HV243_RS04485	932640..933368	-	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
HV243_RS04490	933421..933732	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV243_RS04495	933896..934555	+	660	WP_016154351.1	hemolysin III family protein	-
HV243_RS04500	934635..935615	-	981	WP_053389491.1	tRNA-modifying protein YgfZ	-
HV243_RS04505	935873..936139	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV243_RS04510	936120..936527	+	408	WP_016154348.1	protein YgfX	Toxin
HV243_RS04515	936628..937149	-	522	WP_016154347.1	flavodoxin FldB	-
HV243_RS04520	937263..938159	+	897	WP_046274931.1	site-specific tyrosine recombinase XerD	-
HV243_RS04525	938183..938896	+	714	WP_016154345.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV243_RS04530	938902..940635	+	1734	WP_053389488.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T164011 WP_016154348.1 NZ_CP056717:936120-936527 [Citrobacter sp. RHBSTW-00599]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T164011 NZ_CP072786:172242-172553 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT164011 WP_016154349.1 NZ_CP056717:935873-936139 [Citrobacter sp. RHBSTW-00599]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT164011 NZ_CP072786:172554-172844 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6