Detailed information of TA system TA164008

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4001717..4002396
• Replicon: chromosome
• Accession: NZ_CP056716
• Organism: Enterobacter asburiae strain RHBSTW-00516

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: HV228_RS19645
• Protein ID: WP_006812012.1
• Coordinates: 4001717..4002058 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: HV228_RS19650
• Protein ID: WP_006812013.1
• Coordinates: 4002079..4002396 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV228_RS19630	3998468..4000315	+	1848	WP_023309228.1	RNA polymerase sigma factor RpoD	-
HV228_RS19635	4000848..4001354	-	507	WP_029741755.1	G/U mismatch-specific DNA glycosylase	-
HV228_RS19645	4001717..4002058	-	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
HV228_RS19650	4002079..4002396	-	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV228_RS19655	4002416..4002637	-	222	WP_001548171.1	DUF987 domain-containing protein	-
HV228_RS19660	4002646..4003122	-	477	WP_000811693.1	RadC family protein	-
HV228_RS19665	4003138..4003596	-	459	WP_000211838.1	antirestriction protein	-
HV228_RS19670	4003694..4003933	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV228_RS19675	4004010..4004477	-	468	WP_001547765.1	hypothetical protein	-
HV228_RS19680	4004506..4004943	-	438	WP_016154551.1	hypothetical protein	-
HV228_RS19685	4004943..4005179	-	237	WP_001144031.1	hypothetical protein	-
HV228_RS19690	4005220..4005921	-	702	WP_154916206.1	WYL domain-containing protein	-
HV228_RS19695	4006138..4006956	-	819	WP_181593875.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4001717..4015017	13300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T164008 WP_006812012.1 NZ_CP056716:c4002058-4001717 [Enterobacter asburiae]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T164008 NZ_CP072785:4173485-4173859 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT164008 WP_006812013.1 NZ_CP056716:c4002396-4002079 [Enterobacter asburiae]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT164008 NZ_CP072785:4173027-4173395 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2