Detailed information of TA system TA164005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2943802..2944392
• Replicon: chromosome
• Accession: NZ_CP056716
• Organism: Enterobacter asburiae strain RHBSTW-00516

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV228_RS14525
• Protein ID: WP_181593443.1
• Coordinates: 2943802..2944134 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HV228_RS14530
• Protein ID: WP_033145928.1
• Coordinates: 2944135..2944392 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV228_RS14510	2939966..2941291	+	1326	WP_181593441.1	SidA/IucD/PvdA family monooxygenase	-
HV228_RS14515	2941318..2943507	+	2190	WP_181593442.1	TonB-dependent siderophore receptor	-
HV228_RS14525	2943802..2944134	-	333	WP_181593443.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV228_RS14530	2944135..2944392	-	258	WP_033145928.1	antitoxin	Antitoxin
HV228_RS14535	2944684..2945073	-	390	WP_181593444.1	RidA family protein	-
HV228_RS14540	2945215..2946135	+	921	WP_181595199.1	LysR family transcriptional regulator	-
HV228_RS14545	2946287..2947198	-	912	WP_181593445.1	LysR family transcriptional regulator	-
HV228_RS14550	2947369..2947977	+	609	WP_181593446.1	glutathione S-transferase family protein	-
HV228_RS14555	2948021..2948971	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11826.68 Da
• Isoelectric Point: 9.4051

>T164005 WP_181593443.1 NZ_CP056716:c2944134-2943802 [Enterobacter asburiae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTVGFTVSLEGAGTTTTGVIRCDQPRTI
DMAARNGMRLERIPDAVVNEVLARLDAILN

Nucleotide

• Length: 333 bp

>T164005 NZ_CP072785:c3077421-3077170 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9464.92 Da
• Isoelectric Point: 4.4737

>AT164005 WP_033145928.1 NZ_CP056716:c2944392-2944135 [Enterobacter asburiae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEEREWIDAPAV
GREIL

Nucleotide

• Length: 258 bp

>AT164005 NZ_CP072785:c3077173-3076919 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA