Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1059776..1060396 Replicon chromosome
Accession NZ_CP056716
Organism Enterobacter asburiae strain RHBSTW-00516

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag HV228_RS05115 Protein ID WP_008499287.1
Coordinates 1059776..1059994 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag HV228_RS05120 Protein ID WP_008499288.1
Coordinates 1060022..1060396 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV228_RS05085 1055790..1056050 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
HV228_RS05090 1056053..1056193 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HV228_RS05095 1056190..1056900 - 711 WP_181594513.1 GNAT family N-acetyltransferase -
HV228_RS05100 1057002..1058462 + 1461 WP_181594514.1 PLP-dependent aminotransferase family protein -
HV228_RS05105 1058434..1058901 - 468 WP_023310598.1 hypothetical protein -
HV228_RS05110 1059019..1059570 - 552 WP_181594515.1 maltose O-acetyltransferase -
HV228_RS05115 1059776..1059994 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
HV228_RS05120 1060022..1060396 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
HV228_RS05125 1060907..1064053 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit -
HV228_RS05130 1064076..1065269 - 1194 WP_181594516.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T163996 WP_008499287.1 NZ_CP056716:c1059994-1059776 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T163996 NZ_CP072785:886584-886898 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT163996 WP_008499288.1 NZ_CP056716:c1060396-1060022 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT163996 NZ_CP072785:886348-886581 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References