Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 103760..104286 Replicon plasmid pRHBSTW-00542_2
Accession NZ_CP056712
Organism Enterobacter asburiae strain RHBSTW-00542

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV233_RS24560 Protein ID WP_000323025.1
Coordinates 103999..104286 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV233_RS24555 Protein ID WP_000534858.1
Coordinates 103760..103999 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV233_RS24540 99819..101159 + 1341 WP_181553545.1 ISNCY family transposase -
HV233_RS24545 101802..102374 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HV233_RS24550 102574..103497 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
HV233_RS24555 103760..103999 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV233_RS24560 103999..104286 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV233_RS24565 104358..104516 + 159 WP_008325141.1 type I toxin-antitoxin system Hok family toxin -
HV233_RS24570 105393..105707 + 315 WP_045620735.1 hypothetical protein -
HV233_RS24575 105769..105975 + 207 WP_045337189.1 hypothetical protein -
HV233_RS24580 106053..106514 + 462 WP_049012133.1 hypothetical protein -
HV233_RS24585 106602..106802 + 201 WP_013087182.1 hypothetical protein -
HV233_RS24590 106843..107634 + 792 WP_045620736.1 N-6 DNA methylase -
HV233_RS24595 107676..108004 + 329 Protein_110 hypothetical protein -
HV233_RS24600 108693..108986 + 294 WP_045620738.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..240613 240613
- flank IS/Tn - - 98597..99670 1073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T163994 WP_000323025.1 NZ_CP056712:103999-104286 [Enterobacter asburiae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T163994 NZ_CP072785:775864-776151 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT163994 WP_000534858.1 NZ_CP056712:103760-103999 [Enterobacter asburiae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT163994 NZ_CP072785:776138-776440 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References