Detailed information of TA system TA163991

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3747980..3748659
• Replicon: chromosome
• Accession: NZ_CP056711
• Organism: Enterobacter asburiae strain RHBSTW-00542

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7D6YND6
• Locus tag: HV233_RS18320
• Protein ID: WP_032642920.1
• Coordinates: 3748363..3748659 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A9K1QZR3
• Locus tag: HV233_RS18315
• Protein ID: WP_032642918.1
• Coordinates: 3747980..3748303 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV233_RS18285	3744158..3744427	+	270	WP_009653703.1	metal-sensing transcriptional repressor	-
HV233_RS18290	3744440..3745582	+	1143	WP_029602872.1	nickel/cobalt efflux protein RcnA	-
HV233_RS18295	3745743..3746183	+	441	WP_135695985.1	NirD/YgiW/YdeI family stress tolerance protein	-
HV233_RS18300	3746404..3747225	+	822	WP_032642912.1	DUF945 domain-containing protein	-
HV233_RS18305	3747258..3747728	+	471	WP_032642914.1	DNA repair protein RadC	-
HV233_RS18310	3747737..3747958	+	222	WP_032642916.1	DUF987 domain-containing protein	-
HV233_RS18315	3747980..3748303	+	324	WP_032642918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV233_RS18320	3748363..3748659	+	297	WP_032642920.1	TA system toxin CbtA family protein	Toxin
HV233_RS18325	3749158..3750338	+	1181	Protein_3587	tyrosine-type recombinase/integrase	-
HV233_RS18330	3750375..3751358	-	984	WP_080461224.1	AIPR family protein	-
HV233_RS18335	3751386..3751517	-	132	WP_181553537.1	AIPR family protein	-
HV233_RS18345	3752737..3753603	-	867	WP_181553420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-97)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11228.87 Da
• Isoelectric Point: 6.0673

>T163991 WP_032642920.1 NZ_CP056711:3748363-3748659 [Enterobacter asburiae]
VPVSSRLSPVMVWQQLLTYLLEHHYGLTLNDTPFHDDTAIEEHIDAGITLADAVNFLVEKYELARIDRKGFTWREQPPFI
TATDILRAKKNIGLLPRS

Nucleotide

• Length: 297 bp

>T163991 NZ_CP072785:172242-172553 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12082.82 Da
• Isoelectric Point: 6.6250

>AT163991 WP_032642918.1 NZ_CP056711:3747980-3748303 [Enterobacter asburiae]
MQNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGTFNEEEALRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLYHN
GLTCEADTLGSHGYVYIVIYPQAFATR

Nucleotide

• Length: 324 bp

>AT163991 NZ_CP072785:172554-172844 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A7D6YND6