Detailed information of TA system TA163978

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 83772..84491
• Replicon: chromosome
• Accession: NZ_CP056711
• Organism: Enterobacter asburiae strain RHBSTW-00542

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A144XIR2
• Locus tag: HV233_RS00420
• Protein ID: WP_023305176.1
• Coordinates: 83772..84092 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV233_RS00425
• Protein ID: WP_032620726.1
• Coordinates: 84126..84491 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV233_RS00400	79086..80375	-	1290	WP_181553438.1	RNA-directed DNA polymerase	-
HV233_RS00410	81628..82278	-	651	WP_181553439.1	hypothetical protein	-
HV233_RS00415	82279..83532	-	1254	WP_049110871.1	RNA-directed DNA polymerase	-
HV233_RS00420	83772..84092	-	321	WP_023305176.1	TA system toxin CbtA family protein	Toxin
HV233_RS00425	84126..84491	-	366	WP_032620726.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV233_RS00430	84523..84744	-	222	WP_032620724.1	DUF987 domain-containing protein	-
HV233_RS00435	84753..85223	-	471	WP_032620722.1	DNA repair protein RadC	-
HV233_RS00440	85293..86114	-	822	WP_032620720.1	DUF945 domain-containing protein	-
HV233_RS00445	86557..86748	-	192	WP_023305181.1	hypothetical protein	-
HV233_RS00450	86807..87646	-	840	WP_050595703.1	hypothetical protein	-
HV233_RS00455	87759..88193	-	435	WP_044488933.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	77046..110058	33012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12190.77 Da
• Isoelectric Point: 5.9559

>T163978 WP_023305176.1 NZ_CP056711:c84092-83772 [Enterobacter asburiae]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVHRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T163978 NZ_CP072784:c1377431-1377213 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13574.29 Da
• Isoelectric Point: 6.4673

>AT163978 WP_032620726.1 NZ_CP056711:c84491-84126 [Enterobacter asburiae]
MQSTTISGTSAENTSCLQWELKRNITPCFGARLVQESNRLYFLADRAGFNGSFSDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT163978 NZ_CP072784:c1377831-1377457 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A144XIR2