Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4918..5654 Replicon plasmid pRHBSTW-00564_2
Accession NZ_CP056708
Organism Enterobacter hormaechei strain RHBSTW-00564

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV236_RS22615 Protein ID WP_003026803.1
Coordinates 5172..5654 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV236_RS22610 Protein ID WP_003026799.1
Coordinates 4918..5184 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV236_RS22585 1..1011 + 1011 WP_000200070.1 RepB family plasmid replication initiator protein -
HV236_RS22590 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
HV236_RS22595 2813..3781 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
HV236_RS22600 4026..4229 + 204 WP_004213596.1 hemolysin expression modulator Hha -
HV236_RS22605 4243..4473 + 231 WP_023307222.1 hypothetical protein -
HV236_RS22610 4918..5184 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV236_RS22615 5172..5654 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV236_RS22620 5866..7209 + 1344 WP_080339306.1 ISNCY family transposase -
HV236_RS22625 7280..8098 - 819 WP_023307220.1 abortive infection family protein -
HV236_RS22630 8107..8685 - 579 WP_181522286.1 recombinase family protein -
HV236_RS22635 9118..10238 + 1121 WP_096147877.1 IS3-like element ISSen4 family transposase -
HV236_RS22640 10231..10617 - 387 WP_023307218.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..140774 140774
- inside IScluster/Tn - - 2813..14362 11549


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T163977 WP_003026803.1 NZ_CP056708:5172-5654 [Enterobacter hormaechei]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T163977 NZ_CP072784:1162970-1163368 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT163977 WP_003026799.1 NZ_CP056708:4918-5184 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT163977 NZ_CP072784:1162674-1162967 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References