Detailed information of TA system TA163976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4608937..4609553
• Replicon: chromosome
• Accession: NZ_CP056707
• Organism: Enterobacter hormaechei strain RHBSTW-00564

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV236_RS22165
• Protein ID: WP_077266491.1
• Coordinates: 4608937..4609308 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HV236_RS22170
• Protein ID: WP_015569912.1
• Coordinates: 4609311..4609553 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV236_RS22150	4606437..4607339	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
HV236_RS22155	4607336..4607971	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
HV236_RS22160	4607968..4608897	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
HV236_RS22165	4608937..4609308	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV236_RS22170	4609311..4609553	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV236_RS22175	4609752..4610672	+	921	WP_047051644.1	alpha/beta hydrolase	-
HV236_RS22180	4610681..4611622	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HV236_RS22185	4611667..4612104	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV236_RS22190	4612101..4612982	-	882	WP_181522240.1	virulence factor BrkB family protein	-
HV236_RS22195	4612976..4613575	-	600	WP_032649898.1	glucose-1-phosphatase	-
HV236_RS22200	4613694..4614494	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T163976 WP_077266491.1 NZ_CP056707:c4609308-4608937 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T163976 NZ_CP072784:886584-886898 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT163976 WP_015569912.1 NZ_CP056707:c4609553-4609311 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT163976 NZ_CP072784:886348-886581 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3