Detailed information of TA system TA163958

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 919013..919692
• Replicon: chromosome
• Accession: NZ_CP056707
• Organism: Enterobacter hormaechei strain RHBSTW-00564

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: HV236_RS04360
• Protein ID: WP_000854680.1
• Coordinates: 919013..919354 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A5C1BXL1
• Locus tag: HV236_RS04365
• Protein ID: WP_023336793.1
• Coordinates: 919375..919692 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV236_RS04335	914185..914586	+	402	WP_006809999.1	sigma factor-binding protein Crl	-
HV236_RS04340	914718..915770	-	1053	WP_047052447.1	phosphoporin PhoE	-
HV236_RS04345	916076..917179	+	1104	WP_015571369.1	glutamate 5-kinase	-
HV236_RS04350	917191..918444	+	1254	WP_047052448.1	glutamate-5-semialdehyde dehydrogenase	-
HV236_RS04360	919013..919354	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HV236_RS04365	919375..919692	-	318	WP_023336793.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HV236_RS04370	919711..919932	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HV236_RS04375	919941..920417	-	477	WP_023336794.1	RadC family protein	-
HV236_RS04380	920433..920891	-	459	WP_000211838.1	antirestriction protein	-
HV236_RS04385	920989..921228	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV236_RS04390	921305..921772	-	468	WP_001581459.1	hypothetical protein	-
HV236_RS04395	921796..922239	-	444	WP_000824223.1	hypothetical protein	-
HV236_RS04400	922239..922475	-	237	WP_001144031.1	hypothetical protein	-
HV236_RS04405	922516..923217	-	702	WP_006812588.1	WYL domain-containing protein	-
HV236_RS04410	923434..924255	-	822	WP_000197389.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T163958 WP_000854680.1 NZ_CP056707:c919354-919013 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T163958 NZ_CP072774:236477-236827 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12016.63 Da
• Isoelectric Point: 7.0270

>AT163958 WP_023336793.1 NZ_CP056707:c919692-919375 [Enterobacter hormaechei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT163958 NZ_CP072774:236185-236472 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: A0A5C1BXL1