Detailed information of TA system TA163957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 318897..319514
• Replicon: chromosome
• Accession: NZ_CP056707
• Organism: Enterobacter hormaechei strain RHBSTW-00564

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HV236_RS01535
• Protein ID: WP_181522244.1
• Coordinates: 319191..319514 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A837FG03
• Locus tag: HV236_RS01530
• Protein ID: WP_022650216.1
• Coordinates: 318897..319184 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV236_RS01525	317269..318900	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
HV236_RS01530	318897..319184	-	288	WP_022650216.1	putative addiction module antidote protein	Antitoxin
HV236_RS01535	319191..319514	-	324	WP_181522244.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV236_RS01540	319536..320656	+	1121	WP_085950818.1	IS3-like element ISSen4 family transposase	-
HV236_RS01545	320916..321788	+	873	WP_022646725.1	23S rRNA pseudouridine(2604) synthase RluF	-
HV236_RS01550	321789..322061	-	273	WP_047052050.1	DUF3811 domain-containing protein	-
HV236_RS01555	322112..323056	-	945	WP_032627014.1	ketopantoate/pantoate/pantothenate transporter PanS	-
HV236_RS01560	323150..324499	-	1350	WP_045344657.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (17-100)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11990.85 Da
• Isoelectric Point: 10.6105

>T163957 WP_181522244.1 NZ_CP056707:c319514-319191 [Enterobacter hormaechei]
MKTLLTSGCTNQRGAGHRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVL
LCGGDKSSQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 324 bp

>T163957 NZ_CP072769:2348223-2348960 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACAGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGTTCTCAATTTAGTACGATTATTCCATTAGA
GTACATTGAGCAATTGAATGAGCAGAAAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10087.57 Da
• Isoelectric Point: 5.7807

>AT163957 WP_022650216.1 NZ_CP056707:c319184-318897 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT163957 NZ_CP072769:2349073-2349198 [Bacillus cereus]
GTGATAAAAAATAATGATAATGATTTTTTTAAAGAAGACAATGAAGATACAACGGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGATTTAGAAGATGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A837FG03