Detailed information of TA system TA163953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37141..37938
• Replicon: plasmid pRHBSTW-00577_4
• Accession: NZ_CP056704
• Organism: Klebsiella grimontii strain RHBSTW-00577

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV240_RS29140
• Protein ID: WP_174510223.1
• Coordinates: 37435..37938 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: HV240_RS29135
• Protein ID: WP_016582626.1
• Coordinates: 37141..37410 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV240_RS29110	32492..33832	-	1341	WP_006812582.1	AAA family ATPase	-
HV240_RS29115	33893..34618	-	726	WP_064411599.1	hypothetical protein	-
HV240_RS29120	34981..35658	+	678	WP_064411600.1	hypothetical protein	-
HV240_RS29125	35700..36053	-	354	WP_160956455.1	hypothetical protein	-
HV240_RS29130	36059..36724	-	666	WP_064411601.1	AAA family ATPase	-
HV240_RS29135	37141..37410	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
HV240_RS29140	37435..37938	+	504	WP_174510223.1	GNAT family N-acetyltransferase	Toxin
HV240_RS29145	38123..38374	-	252	WP_064411603.1	hypothetical protein	-
HV240_RS29150	38376..39068	-	693	WP_064411604.1	hypothetical protein	-
HV240_RS29155	39085..39408	-	324	WP_004109805.1	hypothetical protein	-
HV240_RS29160	39508..40941	-	1434	WP_064411605.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112125	112125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18631.35 Da
• Isoelectric Point: 8.8869

>T163953 WP_174510223.1 NZ_CP056704:37435-37938 [Klebsiella grimontii]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGVNANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T163953 NZ_CP072765:3831600-3832010 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTCGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT163953 WP_016582626.1 NZ_CP056704:37141-37410 [Klebsiella grimontii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT163953 NZ_CP072765:3831325-3831603 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3