Detailed information of TA system TA163952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 52624..53360
• Replicon: plasmid pRHBSTW-00577_3
• Accession: NZ_CP056703
• Organism: Klebsiella grimontii strain RHBSTW-00577

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: HV240_RS28475
• Protein ID: WP_004098919.1
• Coordinates: 52624..53106 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: HV240_RS28480
• Protein ID: WP_004098921.1
• Coordinates: 53094..53360 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV240_RS28455	47794..48576	+	783	Protein_50	hypothetical protein	-
HV240_RS28460	48617..49408	-	792	WP_003032641.1	tetratricopeptide repeat protein	-
HV240_RS28465	49467..50813	-	1347	WP_077255298.1	ISNCY family transposase	-
HV240_RS28470	50933..52465	+	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-
HV240_RS28475	52624..53106	-	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
HV240_RS28480	53094..53360	-	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
HV240_RS28485	53735..54703	+	969	WP_163467572.1	IS5 family transposase	-
HV240_RS28490	54932..55189	-	258	WP_004098928.1	hypothetical protein	-
HV240_RS28495	55238..55441	-	204	WP_004098931.1	hemolysin expression modulator Hha	-
HV240_RS28500	55472..55840	-	369	WP_063923539.1	hypothetical protein	-
HV240_RS28505	55884..56378	-	495	WP_013023776.1	hypothetical protein	-
HV240_RS28510	56409..56975	-	567	WP_009654312.1	hypothetical protein	-
HV240_RS28515	56972..57235	-	264	WP_009310051.1	hypothetical protein	-
HV240_RS28520	57525..58103	+	579	WP_009654302.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..133995	133995
	-	inside	IScluster/Tn	-	-	47085..54703	7618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T163952 WP_004098919.1 NZ_CP056703:c53106-52624 [Klebsiella grimontii]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T163952 NZ_CP072765:c3804687-3804295 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT163952 WP_004098921.1 NZ_CP056703:c53360-53094 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163952 NZ_CP072765:c3804962-3804687 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0