Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 51197..51933 Replicon plasmid pRHBSTW-00577_2
Accession NZ_CP056702
Organism Klebsiella grimontii strain RHBSTW-00577

Toxin (Protein)


Gene name tacT Uniprot ID J5UJ49
Locus tag HV240_RS27690 Protein ID WP_009654334.1
Coordinates 51197..51679 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV240_RS27695 Protein ID WP_003026799.1
Coordinates 51667..51933 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV240_RS27670 46685..47411 - 727 Protein_50 ISNCY family transposase -
HV240_RS27675 47522..49054 + 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
HV240_RS27680 49197..49595 - 399 WP_032422684.1 helix-turn-helix domain-containing protein -
HV240_RS27685 49644..50990 - 1347 WP_077255522.1 ISNCY family transposase -
HV240_RS27690 51197..51679 - 483 WP_009654334.1 GNAT family N-acetyltransferase Toxin
HV240_RS27695 51667..51933 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV240_RS27700 52172..52606 - 435 WP_042928448.1 cell envelope integrity protein TolA -
HV240_RS27705 52667..53635 + 969 WP_077267151.1 IS5 family transposase -
HV240_RS27710 53954..54979 - 1026 WP_001101446.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..144021 144021
- inside IScluster/Tn - - 46667..54979 8312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17339.06 Da        Isoelectric Point: 8.7400

>T163951 WP_009654334.1 NZ_CP056702:c51679-51197 [Klebsiella grimontii]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHVKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T163951 NZ_CP072765:3776088-3776345 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT163951 WP_003026799.1 NZ_CP056702:c51933-51667 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT163951 NZ_CP072765:3775816-3776091 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4QCA1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References