Detailed information of TA system TA163950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5848..6373
• Replicon: plasmid pRHBSTW-00577_2
• Accession: NZ_CP056702
• Organism: Klebsiella grimontii strain RHBSTW-00577

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: HV240_RS27455
• Protein ID: WP_013023785.1
• Coordinates: 5848..6153 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: HV240_RS27460
• Protein ID: WP_001568025.1
• Coordinates: 6155..6373 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV240_RS27425	1527..2153	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
HV240_RS27430	2273..2452	+	180	WP_006788217.1	hypothetical protein	-
HV240_RS27435	2924..3718	-	795	WP_004197635.1	site-specific integrase	-
HV240_RS27440	3916..4932	-	1017	WP_017899884.1	hypothetical protein	-
HV240_RS27445	4943..5257	-	315	WP_053389906.1	hypothetical protein	-
HV240_RS27450	5284..5679	-	396	WP_017899885.1	hypothetical protein	-
HV240_RS27455	5848..6153	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV240_RS27460	6155..6373	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV240_RS27465	6543..6788	-	246	Protein_9	hypothetical protein	-
HV240_RS27470	6928..7617	+	690	WP_004193995.1	hypothetical protein	-
HV240_RS27475	7649..8338	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
HV240_RS27480	8775..9023	+	249	WP_000272716.1	hypothetical protein	-
HV240_RS27485	9020..9592	+	573	WP_020805590.1	hypothetical protein	-
HV240_RS27490	9623..10117	+	495	WP_020805591.1	hypothetical protein	-
HV240_RS27495	10161..10529	+	369	WP_004197807.1	hypothetical protein	-
HV240_RS27500	10563..10766	+	204	WP_004197808.1	hemolysin expression modulator Hha	-
HV240_RS27505	10780..10983	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..144021	144021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T163950 WP_013023785.1 NZ_CP056702:c6153-5848 [Klebsiella grimontii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163950 NZ_CP072765:c3713113-3712685 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT163950 WP_001568025.1 NZ_CP056702:c6373-6155 [Klebsiella grimontii]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163950 NZ_CP072765:c3713359-3713117 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8