Detailed information of TA system TA163935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 54700..55301
• Replicon: plasmid pRHBSTW-00605_3
• Accession: NZ_CP056699
• Organism: Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A829IWB6
• Locus tag: HV246_RS24345
• Protein ID: WP_016262419.1
• Coordinates: 54700..55080 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HV246_RS24350
• Protein ID: WP_001190712.1
• Coordinates: 55080..55301 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV246_RS24315	50767..51111	+	345	WP_016262424.1	hypothetical protein	-
HV246_RS24320	51108..51596	+	489	WP_016262423.1	hypothetical protein	-
HV246_RS24325	51593..52087	+	495	WP_062876039.1	dUTP diphosphatase	-
HV246_RS24330	52102..52764	+	663	WP_181479351.1	DUF2829 domain-containing protein	-
HV246_RS24335	52771..53577	+	807	WP_116986849.1	hypothetical protein	-
HV246_RS24340	53564..54664	+	1101	WP_181479352.1	hypothetical protein	-
HV246_RS24345	54700..55080	-	381	WP_016262419.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV246_RS24350	55080..55301	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV246_RS24355	55446..55910	-	465	WP_089697181.1	hypothetical protein	-
HV246_RS24360	55995..56888	-	894	WP_089697183.1	hypothetical protein	-
HV246_RS24365	57174..57350	-	177	WP_157942684.1	hypothetical protein	-
HV246_RS24370	57350..57643	-	294	WP_089697186.1	hypothetical protein	-
HV246_RS24375	57656..58057	-	402	WP_086351779.1	hypothetical protein	-
HV246_RS24380	58234..58464	-	231	Protein_60	DUF551 domain-containing protein	-
HV246_RS24385	59083..59289	-	207	Protein_61	DUF551 domain-containing protein	-
HV246_RS24390	59300..59755	-	456	WP_032141438.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94137	94137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13678.55 Da
• Isoelectric Point: 5.6406

>T163935 WP_016262419.1 NZ_CP056699:c55080-54700 [Escherichia marmotae]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI

Nucleotide

• Length: 381 bp

>T163935 NZ_CP072765:2813307-2813732 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT163935 WP_001190712.1 NZ_CP056699:c55301-55080 [Escherichia marmotae]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT163935 NZ_CP072765:2813080-2813301 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Toxin

• AlphaFold DB: A0A829IWB6

Antitoxin

• AlphaFold DB: A0A829CJB6