Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 44138..44901 Replicon plasmid pRHBSTW-00605_2
Accession NZ_CP056698
Organism Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)


Gene name tacT Uniprot ID A0A7W3ANZ2
Locus tag HV246_RS23550 Protein ID WP_001558270.1
Coordinates 44138..44620 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7L6LG35
Locus tag HV246_RS23555 Protein ID WP_001558271.1
Coordinates 44611..44901 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV246_RS23505 39419..39514 + 96 Protein_37 hypothetical protein -
HV246_RS23510 39600..40025 + 426 WP_181479337.1 transposase -
HV246_RS23515 40022..40162 + 141 WP_181479350.1 IS66 family insertion sequence element accessory protein TnpB -
HV246_RS23520 40155..40649 + 495 Protein_40 IS66 family transposase -
HV246_RS23525 40862..41149 - 288 WP_024195838.1 helix-turn-helix transcriptional regulator -
HV246_RS23530 41149..41460 - 312 WP_000483534.1 type II toxin-antitoxin system RelE/ParE family toxin -
HV246_RS23535 42434..43291 - 858 WP_021555340.1 incFII family plasmid replication initiator RepA -
HV246_RS23540 43284..43358 - 75 WP_011387702.1 RepA leader peptide Tap -
HV246_RS23545 43593..43853 - 261 WP_000078108.1 replication regulatory protein RepA -
HV246_RS23550 44138..44620 - 483 WP_001558270.1 GNAT family N-acetyltransferase Toxin
HV246_RS23555 44611..44901 - 291 WP_001558271.1 DUF1778 domain-containing protein Antitoxin
HV246_RS23560 45232..45381 - 150 WP_001312851.1 type I toxin-antitoxin system Hok family toxin -
- 45428..45486 + 59 NuclAT_1 - -
- 45428..45486 + 59 NuclAT_1 - -
- 45428..45486 + 59 NuclAT_1 - -
- 45428..45486 + 59 NuclAT_1 - -
HV246_RS23565 46429..47028 - 600 Protein_49 hypothetical protein -
HV246_RS23570 47411..47611 - 201 WP_001558275.1 hypothetical protein -
HV246_RS23575 47744..48304 - 561 WP_001558276.1 fertility inhibition protein FinO -
HV246_RS23580 48378..49136 - 759 WP_021555343.1 type-F conjugative transfer system pilin acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..127911 127911


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(11-95)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17746.49 Da        Isoelectric Point: 6.5974

>T163926 WP_001558270.1 NZ_CP056698:c44620-44138 [Escherichia marmotae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI

Download         Length: 483 bp

>T163926 NZ_CP072765:2204649-2205026 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 11155.62 Da        Isoelectric Point: 4.8896

>AT163926 WP_001558271.1 NZ_CP056698:c44901-44611 [Escherichia marmotae]
MSTAASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQ
NAEGRERLMAVKPEWK

Download         Length: 291 bp

>AT163926 NZ_CP072765:2205068-2205517 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3ANZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LG35

References