Detailed information of TA system TA163924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 7877..8402
• Replicon: plasmid pRHBSTW-00605_2
• Accession: NZ_CP056698
• Organism: Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HV246_RS23360
• Protein ID: WP_001159866.1
• Coordinates: 7877..8182 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A7W3G1F7
• Locus tag: HV246_RS23365
• Protein ID: WP_000813629.1
• Coordinates: 8184..8402 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV246_RS23335	3767..4438	-	672	WP_024188661.1	response regulator transcription factor HprR	-
HV246_RS23340	4570..4983	+	414	WP_000920117.1	hydroxyisourate hydrolase	-
HV246_RS23345	5082..6074	+	993	WP_181479326.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
HV246_RS23350	6075..6710	+	636	WP_001559859.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
HV246_RS23355	7070..7876	-	807	WP_000016962.1	site-specific integrase	-
HV246_RS23360	7877..8182	-	306	WP_001159866.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV246_RS23365	8184..8402	-	219	WP_000813629.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV246_RS23370	8957..12763	-	3807	WP_001559860.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127911	127911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11673.44 Da
• Isoelectric Point: 6.2213

>T163924 WP_001159866.1 NZ_CP056698:c8182-7877 [Escherichia marmotae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAHLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163924 NZ_CP072765:1969489-1969737 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT163924 WP_000813629.1 NZ_CP056698:c8402-8184 [Escherichia marmotae]
MKQRITVTVDSDSYKLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163924 NZ_CP072765:1969277-1969489 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W3G1F7