Detailed information of TA system TA163922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3808006..3808784
• Replicon: chromosome
• Accession: NZ_CP056697
• Organism: Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HV246_RS18200
• Protein ID: WP_001517064.1
• Coordinates: 3808006..3808494 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7H9KCB5
• Locus tag: HV246_RS18205
• Protein ID: WP_001110459.1
• Coordinates: 3808491..3808784 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV246_RS18195	3805747..3807111	+	1365	Protein_3549	EAL domain-containing protein	-
HV246_RS18200	3808006..3808494	-	489	WP_001517064.1	GNAT family N-acetyltransferase	Toxin
HV246_RS18205	3808491..3808784	-	294	WP_001110459.1	DUF1778 domain-containing protein	Antitoxin
HV246_RS18210	3809319..3809816	-	498	WP_016249187.1	type VI secretion system contractile sheath small subunit	-
HV246_RS18215	3810408..3810881	+	474	WP_001517065.1	hypothetical protein	-
HV246_RS18220	3810878..3811213	+	336	WP_181478621.1	hypothetical protein	-
HV246_RS18225	3812039..3813022	+	984	WP_001325875.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	clpV/tssH / hcp/tssD / vipB/tssC / vipA/tssB / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / draA / vipA/tssB / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE	3688734..3944062	255328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17521.41 Da
• Isoelectric Point: 7.7535

>T163922 WP_001517064.1 NZ_CP056697:c3808494-3808006 [Escherichia marmotae]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T163922 NZ_CP072765:1766550-1766954 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 9.9171

>AT163922 WP_001110459.1 NZ_CP056697:c3808784-3808491 [Escherichia marmotae]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT163922 NZ_CP072765:1766341-1766544 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H9KCB5