Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 280623..281119 Replicon chromosome
Accession NZ_CP056697
Organism Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)


Gene name ghoT Uniprot ID A0A7U9DCZ4
Locus tag HV246_RS01270 Protein ID WP_001173345.1
Coordinates 280946..281119 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A7U9DAJ1
Locus tag HV246_RS01265 Protein ID WP_001517605.1
Coordinates 280623..280919 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV246_RS01245 277355..278074 - 720 WP_000611294.1 two-component system response regulator DcuR -
HV246_RS01250 278071..279702 - 1632 WP_038355161.1 sensor histidine kinase -
HV246_RS01255 279881..280111 + 231 WP_000371696.1 (4Fe-4S)-binding protein -
HV246_RS01260 280123..280395 + 273 WP_000405652.1 N-acetyltransferase -
HV246_RS01265 280623..280919 + 297 WP_001517605.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HV246_RS01270 280946..281119 + 174 WP_001173345.1 type V toxin-antitoxin system toxin GhoT Toxin
HV246_RS01275 281238..282755 - 1518 WP_001295074.1 lysine--tRNA ligase -
HV246_RS01280 282992..284449 - 1458 WP_001517606.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 280623..303781 23158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T163904 WP_001173345.1 NZ_CP056697:280946-281119 [Escherichia marmotae]
MALFSKILIFYVIGVNISFVIIWFLSHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T163904 NZ_CP072765:703489-703890 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11568.18 Da        Isoelectric Point: 4.0994

>AT163904 WP_001517605.1 NZ_CP056697:280623-280919 [Escherichia marmotae]
MEGINRFKTYVVSFDYPSSYYSVFLRLRSLMYDMDFSSIVADEYGIPRQLNENAFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMIVDDYFRQFYK

Download         Length: 297 bp

>AT163904 NZ_CP072765:703247-703492 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DCZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAJ1

References