Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 42322..43058 Replicon plasmid pRHBSTW-00634_4
Accession NZ_CP056685
Organism Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)


Gene name tacT Uniprot ID A0A486JQ35
Locus tag HV252_RS30195 Protein ID WP_049109282.1
Coordinates 42322..42804 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV252_RS30200 Protein ID WP_003026799.1
Coordinates 42792..43058 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV252_RS30180 38456..39088 + 633 WP_001567369.1 hypothetical protein -
HV252_RS30185 39117..40520 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV252_RS30190 40632..42164 + 1533 WP_181511922.1 IS3 family transposase -
HV252_RS30195 42322..42804 - 483 WP_049109282.1 GNAT family N-acetyltransferase Toxin
HV252_RS30200 42792..43058 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV252_RS30205 43344..43451 + 108 Protein_35 transposase -
HV252_RS30210 43494..44309 - 816 WP_049109278.1 hypothetical protein -
HV252_RS30215 44587..45510 + 924 WP_049108798.1 IS5 family transposase -
HV252_RS30220 45856..47088 + 1233 WP_117103017.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..128344 128344
- inside IScluster/Tn - - 23345..45510 22165


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17336.86 Da        Isoelectric Point: 8.0020

>T163888 WP_049109282.1 NZ_CP056685:c42804-42322 [Klebsiella grimontii]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCETGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T163888 NZ_CP072764:3182818-3183081 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGCATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT163888 WP_003026799.1 NZ_CP056685:c43058-42792 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT163888 NZ_CP072764:3182533-3182814 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486JQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References