Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 51567..52303 Replicon plasmid pRHBSTW-00634_3
Accession NZ_CP056684
Organism Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)


Gene name tacT Uniprot ID J5UJ49
Locus tag HV252_RS29550 Protein ID WP_009654334.1
Coordinates 51821..52303 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV252_RS29545 Protein ID WP_003026799.1
Coordinates 51567..51833 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV252_RS29530 48521..49546 + 1026 WP_001101446.1 IS110 family transposase -
HV252_RS29535 49865..50833 - 969 WP_077266288.1 IS5 family transposase -
HV252_RS29540 50894..51328 + 435 WP_042928448.1 cell envelope integrity protein TolA -
HV252_RS29545 51567..51833 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV252_RS29550 51821..52303 + 483 WP_009654334.1 GNAT family N-acetyltransferase Toxin
HV252_RS29555 52461..53993 - 1533 WP_009310015.1 IS3-like element ISKpn38 family transposase -
HV252_RS29560 54105..55508 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV252_RS29565 55537..56169 - 633 WP_001567369.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..134986 134986
- inside IScluster/Tn - - 48521..64816 16295


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17339.06 Da        Isoelectric Point: 8.7400

>T163887 WP_009654334.1 NZ_CP056684:51821-52303 [Klebsiella grimontii]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHVKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T163887 NZ_CP072764:c3142008-3141616 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT163887 WP_003026799.1 NZ_CP056684:51567-51833 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT163887 NZ_CP072764:c3142220-3142005 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4QCA1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References