Detailed information of TA system TA163886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 92318..93228
• Replicon: plasmid pRHBSTW-00634_2
• Accession: NZ_CP056683
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2A5MBW7
• Locus tag: HV252_RS28645
• Protein ID: WP_023329005.1
• Coordinates: 92318..92788 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV252_RS28650
• Protein ID: WP_023329004.1
• Coordinates: 92785..93228 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS28620	88009..88977	-	969	WP_181507338.1	IS5 family transposase	-
HV252_RS28625	89265..89513	+	249	WP_004187025.1	plasmid stabilization protein	-
HV252_RS28630	89503..89787	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV252_RS28635	89803..91146	-	1344	WP_023329007.1	ISNCY family transposase	-
HV252_RS28640	91948..92207	+	260	Protein_101	hypothetical protein	-
HV252_RS28645	92318..92788	-	471	WP_023329005.1	RES family NAD+ phosphorylase	Toxin
HV252_RS28650	92785..93228	-	444	WP_023329004.1	DUF2384 domain-containing protein	Antitoxin
HV252_RS28655	93350..94037	-	688	Protein_104	DUF4113 domain-containing protein	-
HV252_RS28660	94198..95193	-	996	WP_023329002.1	DUF2891 domain-containing protein	-
HV252_RS28665	95203..96189	-	987	Protein_106	DUF979 domain-containing protein	-
HV252_RS28670	96186..96907	-	722	Protein_107	DUF969 domain-containing protein	-
HV252_RS28675	97106..97444	+	339	WP_032738440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..219226	219226
	-	flank	IS/Tn	-	-	88009..88977	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17459.70 Da
• Isoelectric Point: 4.4829

>T163886 WP_023329005.1 NZ_CP056683:c92788-92318 [Klebsiella grimontii]
VILYRLTKTKNLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPANWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSDFYGIVQMAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T163886 NZ_CP072764:c3139589-3138705 [Mycobacterium tuberculosis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16477.82 Da
• Isoelectric Point: 10.2498

>AT163886 WP_023329004.1 NZ_CP056683:c93228-92785 [Klebsiella grimontii]
MKTFSLSSTPARPQRLWQVAGLNNADSVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAALKWLNEPNRALSWKVPADLIASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT163886 NZ_CP072764:c3140479-3139592 [Mycobacterium tuberculosis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGCCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MBW7