Detailed information of TA system TA163885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 89265..89787
• Replicon: plasmid pRHBSTW-00634_2
• Accession: NZ_CP056683
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: HV252_RS28630
• Protein ID: WP_004187019.1
• Coordinates: 89503..89787 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HV252_RS28625
• Protein ID: WP_004187025.1
• Coordinates: 89265..89513 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS28605	85119..85676	-	558	WP_023329012.1	HutD family protein	-
HV252_RS28610	85673..86434	-	762	WP_023329011.1	histidine utilization repressor	-
HV252_RS28615	86537..87904	+	1368	WP_023329010.1	formimidoylglutamate deiminase	-
HV252_RS28620	88009..88977	-	969	WP_181507338.1	IS5 family transposase	-
HV252_RS28625	89265..89513	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HV252_RS28630	89503..89787	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV252_RS28635	89803..91146	-	1344	WP_023329007.1	ISNCY family transposase	-
HV252_RS28640	91948..92207	+	260	Protein_101	hypothetical protein	-
HV252_RS28645	92318..92788	-	471	WP_023329005.1	RES family NAD+ phosphorylase	-
HV252_RS28650	92785..93228	-	444	WP_023329004.1	DUF2384 domain-containing protein	-
HV252_RS28655	93350..94037	-	688	Protein_104	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..219226	219226
	-	flank	IS/Tn	-	-	88009..88977	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T163885 WP_004187019.1 NZ_CP056683:89503-89787 [Klebsiella grimontii]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T163885 NZ_CP072764:c3115519-3115163 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT163885 WP_004187025.1 NZ_CP056683:89265-89513 [Klebsiella grimontii]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT163885 NZ_CP072764:c3115733-3115503 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4