Detailed information of TA system TA163884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 72290..73026
• Replicon: plasmid pRHBSTW-00634_2
• Accession: NZ_CP056683
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV252_RS28530
• Protein ID: WP_023329018.1
• Coordinates: 72290..72772 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HV252_RS28535
• Protein ID: WP_003026799.1
• Coordinates: 72760..73026 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS28515	68424..69056	+	633	WP_001567369.1	hypothetical protein	-
HV252_RS28520	69085..70488	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
HV252_RS28525	70600..72132	+	1533	WP_004118819.1	IS3 family transposase	-
HV252_RS28530	72290..72772	-	483	WP_023329018.1	GNAT family N-acetyltransferase	Toxin
HV252_RS28535	72760..73026	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HV252_RS28540	73559..73747	+	189	Protein_81	MFS transporter	-
HV252_RS28545	74130..74309	+	180	Protein_82	transposase	-
HV252_RS28550	74276..74410	+	135	WP_109241503.1	integrase core domain-containing protein	-
HV252_RS28555	74562..75371	-	810	WP_023329017.1	N-formylglutamate deformylase	-
HV252_RS28560	75364..76572	-	1209	WP_109233418.1	imidazolonepropionase	-
HV252_RS28565	76584..77978	-	1395	WP_023329015.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..219226	219226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17255.84 Da
• Isoelectric Point: 7.8840

>T163884 WP_023329018.1 NZ_CP056683:c72772-72290 [Klebsiella grimontii]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAEGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T163884 NZ_CP072764:c3076266-3075871 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT163884 WP_003026799.1 NZ_CP056683:c73026-72760 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163884 NZ_CP072764:c3075845-3075579 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66