Detailed information of TA system TA163871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1959749..1960339
• Replicon: chromosome
• Accession: NZ_CP056682
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV252_RS09290
• Protein ID: WP_181511749.1
• Coordinates: 1960007..1960339 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: HV252_RS09285
• Protein ID: WP_000288812.1
• Coordinates: 1959749..1960006 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS09270	1957075..1957854	-	780	WP_181511877.1	hypothetical protein	-
HV252_RS09275	1958736..1959197	+	462	WP_181511747.1	hypothetical protein	-
HV252_RS09280	1959194..1959400	+	207	WP_181511748.1	helix-turn-helix domain-containing protein	-
HV252_RS09285	1959749..1960006	+	258	WP_000288812.1	antitoxin	Antitoxin
HV252_RS09290	1960007..1960339	+	333	WP_181511749.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV252_RS09295	1960386..1960619	-	234	WP_181511908.1	hypothetical protein	-
HV252_RS09300	1961189..1962058	+	870	WP_181511750.1	integrase domain-containing protein	-
HV252_RS09305	1962917..1964179	-	1263	WP_181511751.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11769.61 Da
• Isoelectric Point: 10.1839

>T163871 WP_181511749.1 NZ_CP056682:1960007-1960339 [Klebsiella grimontii]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGSFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMEARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T163871 NZ_CP072764:c2237579-2237235 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT163871 WP_000288812.1 NZ_CP056682:1959749-1960006 [Klebsiella grimontii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT163871 NZ_CP072764:c2237821-2237573 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0