Detailed information of TA system TA163869

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 885907..886679
• Replicon: chromosome
• Accession: NZ_CP056682
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ICB7
• Locus tag: HV252_RS04340
• Protein ID: WP_049238634.1
• Coordinates: 886290..886679 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV252_RS04335
• Protein ID: WP_171547612.1
• Coordinates: 885907..886233 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS04305	882277..882909	-	633	WP_049238458.1	inovirus Gp2 family protein	-
HV252_RS04310	883522..884346	+	825	WP_049238647.1	DUF945 domain-containing protein	-
HV252_RS04315	884343..884600	+	258	WP_049238645.1	DUF905 domain-containing protein	-
HV252_RS04320	884680..885123	+	444	WP_049238643.1	antirestriction protein	-
HV252_RS04325	885135..885614	+	480	WP_049238640.1	DNA repair protein RadC	-
HV252_RS04330	885628..885849	+	222	WP_049238638.1	DUF987 domain-containing protein	-
HV252_RS04335	885907..886233	+	327	WP_171547612.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV252_RS04340	886290..886679	+	390	WP_049238634.1	TA system toxin CbtA family protein	Toxin
HV252_RS04345	886803..887497	+	695	WP_087786203.1	IS1-like element IS1N family transposase	-
HV252_RS04350	887540..888379	+	840	WP_049238632.1	DUF4942 domain-containing protein	-
HV252_RS04355	888712..889062	+	351	WP_004134443.1	transcriptional regulator	-
HV252_RS04360	889115..890407	+	1293	WP_181511676.1	arsenic transporter	-
HV252_RS04365	890417..890842	+	426	WP_004134449.1	glutaredoxin-dependent arsenate reductase	-
HV252_RS04370	891035..891106	+	72	Protein_858	adenylyl-sulfate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14387.45 Da
• Isoelectric Point: 9.6254

>T163869 WP_049238634.1 NZ_CP056682:886290-886679 [Klebsiella grimontii]
MQTKLSPLMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDEQVIQQHIDAGISLSDALNFIVEKYELVRTDRPGF
SIREQSPFITPIDILRARKAAGLMNRGTYKEVTAITRGQHPQANASGKR

Nucleotide

• Length: 390 bp

>T163869 NZ_CP072764:c2228762-2228343 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12174.84 Da
• Isoelectric Point: 6.4673

>AT163869 WP_171547612.1 NZ_CP056682:885907-886233 [Klebsiella grimontii]
ISEPWWGLRRSVSPCFGARLVQERNSLHYLADRANFDGQFCDADLRYLDQAFPVLMKQLELMLTSGELNPRHQHGVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVTTA

Nucleotide

• Length: 327 bp

>AT163869 NZ_CP072764:c2229031-2228771 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A7L6ICB7