Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 23444..24180 Replicon plasmid pRHBSTW-00642_3
Accession NZ_CP056678
Organism Enterobacter hormaechei strain RHBSTW-00642

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV254_RS25130 Protein ID WP_003026803.1
Coordinates 23698..24180 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV254_RS25125 Protein ID WP_003026799.1
Coordinates 23444..23710 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV254_RS25085 19009..20010 + 1002 WP_032635019.1 Abi family protein -
HV254_RS25090 20149..20727 - 579 WP_032635068.1 hypothetical protein -
HV254_RS25095 21017..21280 + 264 WP_009310051.1 hypothetical protein -
HV254_RS25100 21277..21843 + 567 WP_009310052.1 hypothetical protein -
HV254_RS25105 21874..22368 + 495 WP_053504376.1 hypothetical protein -
HV254_RS25110 22429..22632 + 204 WP_004150739.1 hemolysin expression modulator Hha -
HV254_RS25115 22681..22938 + 258 WP_004098928.1 hypothetical protein -
HV254_RS25120 23014..23268 + 255 WP_016528790.1 hypothetical protein -
HV254_RS25125 23444..23710 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV254_RS25130 23698..24180 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV254_RS25135 24392..25735 + 1344 WP_080339306.1 ISNCY family transposase -
HV254_RS25140 25806..26624 - 819 WP_023307220.1 abortive infection family protein -
HV254_RS25145 26633..27205 - 573 WP_032676095.1 recombinase family protein -
HV254_RS25150 27493..27903 - 411 WP_053504377.1 hypothetical protein -
HV254_RS25155 28059..28499 + 441 WP_023307217.1 IS66 family insertion sequence hypothetical protein -
HV254_RS25160 28496..28846 + 351 WP_004189161.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkF / mrkD / mrkC / mrkB / mrkA 1..87741 87741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T163866 WP_003026803.1 NZ_CP056678:23698-24180 [Enterobacter hormaechei]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T163866 NZ_CP072764:c2197902-2197573 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT163866 WP_003026799.1 NZ_CP056678:23444-23710 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT163866 NZ_CP072764:c2198276-2197899 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References