Detailed information of TA system TA163865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 17178..17703
• Replicon: plasmid pRHBSTW-00642_3
• Accession: NZ_CP056678
• Organism: Enterobacter hormaechei strain RHBSTW-00642

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: HV254_RS25070
• Protein ID: WP_016946795.1
• Coordinates: 17178..17483 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HV254_RS25075
• Protein ID: WP_004197642.1
• Coordinates: 17485..17703 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV254_RS25040	12997..13266	+	270	WP_000339857.1	hypothetical protein	-
HV254_RS25045	13622..14488	-	867	WP_004118283.1	replication initiation protein	-
HV254_RS25050	15023..15127	+	105	WP_032409716.1	hypothetical protein	-
HV254_RS25055	15256..15513	+	258	WP_000764642.1	hypothetical protein	-
HV254_RS25060	15571..16347	-	777	WP_049245386.1	site-specific integrase	-
HV254_RS25065	16350..17033	-	684	WP_023280892.1	hypothetical protein	-
HV254_RS25070	17178..17483	-	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV254_RS25075	17485..17703	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV254_RS25080	18272..18853	+	582	WP_009654315.1	hypothetical protein	-
HV254_RS25085	19009..20010	+	1002	WP_032635019.1	Abi family protein	-
HV254_RS25090	20149..20727	-	579	WP_032635068.1	hypothetical protein	-
HV254_RS25095	21017..21280	+	264	WP_009310051.1	hypothetical protein	-
HV254_RS25100	21277..21843	+	567	WP_009310052.1	hypothetical protein	-
HV254_RS25105	21874..22368	+	495	WP_053504376.1	hypothetical protein	-
HV254_RS25110	22429..22632	+	204	WP_004150739.1	hemolysin expression modulator Hha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	mrkF / mrkD / mrkC / mrkB / mrkA	1..87741	87741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T163865 WP_016946795.1 NZ_CP056678:c17483-17178 [Enterobacter hormaechei]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163865 NZ_CP072764:1969488-1969736 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT163865 WP_004197642.1 NZ_CP056678:c17703-17485 [Enterobacter hormaechei]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163865 NZ_CP072764:1969276-1969488 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA