Detailed information of TA system TA163836

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4415478..4416157
• Replicon: chromosome
• Accession: NZ_CP056647
• Organism: Citrobacter sp. RHBSTW-00229

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: HV135_RS21330
• Protein ID: WP_006812012.1
• Coordinates: 4415478..4415819 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: HV135_RS21335
• Protein ID: WP_006812013.1
• Coordinates: 4415840..4416157 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV135_RS21310	4410809..4412554	+	1746	WP_019077713.1	DNA primase	-
HV135_RS21315	4412719..4414563	+	1845	WP_016154549.1	RNA polymerase sigma factor RpoD	-
HV135_RS21320	4414609..4415115	-	507	WP_103767095.1	G/U mismatch-specific DNA glycosylase	-
HV135_RS21330	4415478..4415819	-	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
HV135_RS21335	4415840..4416157	-	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV135_RS21340	4416177..4416398	-	222	WP_001548171.1	DUF987 domain-containing protein	-
HV135_RS21345	4416407..4416883	-	477	WP_000811693.1	RadC family protein	-
HV135_RS21350	4416899..4417357	-	459	WP_000211838.1	antirestriction protein	-
HV135_RS21355	4417455..4417694	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV135_RS21360	4417771..4418238	-	468	WP_001547765.1	hypothetical protein	-
HV135_RS21365	4418264..4418704	-	441	WP_049040828.1	hypothetical protein	-
HV135_RS21370	4418704..4418940	-	237	WP_001144031.1	hypothetical protein	-
HV135_RS21375	4418981..4419682	-	702	WP_001581462.1	WYL domain-containing protein	-
HV135_RS21380	4419899..4420588	-	690	Protein_4185	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4415478..4438783	23305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T163836 WP_006812012.1 NZ_CP056647:c4415819-4415478 [Citrobacter sp. RHBSTW-00229]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T163836 NZ_CP072764:71818-72219 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT163836 WP_006812013.1 NZ_CP056647:c4416157-4415840 [Citrobacter sp. RHBSTW-00229]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT163836 NZ_CP072764:71586-71825 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2