Detailed information of TA system TA163835

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4193820..4194474
• Replicon: chromosome
• Accession: NZ_CP056647
• Organism: Citrobacter sp. RHBSTW-00229

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV135_RS20225
• Protein ID: WP_016154348.1
• Coordinates: 4193820..4194227 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV135_RS20230
• Protein ID: WP_016154349.1
• Coordinates: 4194208..4194474 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV135_RS20205	4189712..4191445	-	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-
HV135_RS20210	4191451..4192164	-	714	WP_103766660.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV135_RS20215	4192188..4193084	-	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV135_RS20220	4193198..4193719	+	522	WP_016154347.1	flavodoxin FldB	-
HV135_RS20225	4193820..4194227	-	408	WP_016154348.1	protein YgfX	Toxin
HV135_RS20230	4194208..4194474	-	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV135_RS20235	4194732..4195712	+	981	WP_181220817.1	tRNA-modifying protein YgfZ	-
HV135_RS20240	4195792..4196451	-	660	WP_153690042.1	hemolysin III family protein	-
HV135_RS20245	4196615..4196926	-	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV135_RS20250	4196979..4197707	+	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
HV135_RS20255	4197829..4199262	+	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T163835 WP_016154348.1 NZ_CP056647:c4194227-4193820 [Citrobacter sp. RHBSTW-00229]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T163835 NZ_CP072763:3831600-3832010 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTCGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT163835 WP_016154349.1 NZ_CP056647:c4194474-4194208 [Citrobacter sp. RHBSTW-00229]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT163835 NZ_CP072763:3831325-3831603 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6