Detailed information of TA system TA163825

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1438951..1439630
• Replicon: chromosome
• Accession: NZ_CP056647
• Organism: Citrobacter sp. RHBSTW-00229

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV135_RS06840
• Protein ID: WP_131347937.1
• Coordinates: 1438951..1439292 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV135_RS06845
• Protein ID: WP_131347939.1
• Coordinates: 1439313..1439630 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV135_RS06805	1434056..1434592	+	537	WP_047501855.1	fimbria assembly protein	-
HV135_RS06810	1434642..1435274	-	633	WP_016152131.1	fimbria biosynthesis transcriptional regulator FimZ	-
HV135_RS06815	1435862..1436584	-	723	WP_047501853.1	hypothetical protein	-
HV135_RS06820	1436970..1437563	-	594	WP_181220707.1	fimbria biosynthesis transcriptional regulator FimW	-
HV135_RS06825	1437601..1437780	+	180	WP_016152134.1	hypothetical protein	-
HV135_RS06835	1437999..1438835	-	837	WP_131347935.1	DUF4942 domain-containing protein	-
HV135_RS06840	1438951..1439292	-	342	WP_131347937.1	TA system toxin CbtA family protein	Toxin
HV135_RS06845	1439313..1439630	-	318	WP_131347939.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV135_RS06850	1439649..1439870	-	222	WP_023326102.1	DUF987 domain-containing protein	-
HV135_RS06855	1439879..1440355	-	477	WP_000811693.1	RadC family protein	-
HV135_RS06860	1440371..1440829	-	459	WP_000211838.1	antirestriction protein	-
HV135_RS06865	1440927..1441166	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV135_RS06870	1441243..1441710	-	468	WP_001547765.1	hypothetical protein	-
HV135_RS06875	1441733..1442176	-	444	WP_131347941.1	hypothetical protein	-
HV135_RS06880	1442176..1442412	-	237	WP_045341362.1	hypothetical protein	-
HV135_RS06885	1442453..1443154	-	702	Protein_1343	WYL domain-containing protein	-
HV135_RS06890	1443371..1444060	-	690	Protein_1344	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12928.91 Da
• Isoelectric Point: 10.2764

>T163825 WP_131347937.1 NZ_CP056647:c1439292-1438951 [Citrobacter sp. RHBSTW-00229]
MKPQSAATSRAVKPCLTPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRKNRNNAVR

Nucleotide

• Length: 342 bp

>T163825 NZ_CP072763:c3076267-3075872 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11968.69 Da
• Isoelectric Point: 7.3232

>AT163825 WP_131347939.1 NZ_CP056647:c1439630-1439313 [Citrobacter sp. RHBSTW-00229]
MSNITWGLRRDITPRLGARLVQEGNRLHYLADRASMTGKFSDAECQKLDETFPHFIRQMESMLTTGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT163825 NZ_CP072763:c3075846-3075580 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA