Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1345052..1345672 Replicon chromosome
Accession NZ_CP056647
Organism Citrobacter sp. RHBSTW-00229

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV135_RS06410 Protein ID WP_002892050.1
Coordinates 1345052..1345270 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7L6U5G9
Locus tag HV135_RS06415 Protein ID WP_019076175.1
Coordinates 1345298..1345672 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV135_RS06375 1340411..1341040 + 630 WP_016155872.1 membrane protein -
HV135_RS06380 1341105..1341458 + 354 WP_003831033.1 DUF1428 family protein -
HV135_RS06385 1341510..1343063 - 1554 WP_047501906.1 EAL domain-containing protein -
HV135_RS06390 1343252..1343512 + 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV135_RS06395 1343514..1343654 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV135_RS06400 1343733..1344203 - 471 WP_047416865.1 YlaC family protein -
HV135_RS06405 1344320..1344871 - 552 WP_181220739.1 maltose O-acetyltransferase -
HV135_RS06410 1345052..1345270 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV135_RS06415 1345298..1345672 - 375 WP_019076175.1 Hha toxicity modulator TomB Antitoxin
HV135_RS06420 1346160..1349309 - 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV135_RS06425 1349332..1350525 - 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163824 WP_002892050.1 NZ_CP056647:c1345270-1345052 [Citrobacter sp. RHBSTW-00229]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163824 NZ_CP072763:c3075583-3075167 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14413.20 Da        Isoelectric Point: 5.5653

>AT163824 WP_019076175.1 NZ_CP056647:c1345672-1345298 [Citrobacter sp. RHBSTW-00229]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163824 NZ_CP072763:c3075846-3075580 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U5G9

References