Detailed information of TA system TA163819

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 62103..62856
• Replicon: plasmid pRHBSTW-00267_2
• Accession: NZ_CP056645
• Organism: Citrobacter freundii strain RHBSTW-00267

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A097QYR4
• Locus tag: HV139_RS24000
• Protein ID: WP_025799003.1
• Coordinates: 62103..62480 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7T0E0C6
• Locus tag: HV139_RS24005
• Protein ID: WP_072269511.1
• Coordinates: 62530..62856 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV139_RS23965	57342..57656	-	315	WP_009652923.1	hypothetical protein	-
HV139_RS23970	58520..59482	+	963	WP_007372199.1	plasmid stabilization protein	-
HV139_RS23975	59496..59882	+	387	WP_009652914.1	plasmid stability protein	-
HV139_RS23980	59909..60313	-	405	WP_007372197.1	FCD domain-containing protein	-
HV139_RS23985	60456..61301	-	846	WP_049016442.1	DUF4942 domain-containing protein	-
HV139_RS23990	61383..61586	-	204	WP_025799007.1	DUF957 domain-containing protein	-
HV139_RS23995	61615..62106	-	492	WP_045888216.1	hypothetical protein	-
HV139_RS24000	62103..62480	-	378	WP_025799003.1	TA system toxin CbtA family protein	Toxin
HV139_RS24005	62530..62856	-	327	WP_072269511.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV139_RS24010	62913..63134	-	222	WP_045888217.1	DUF987 domain-containing protein	-
HV139_RS24015	63743..64894	-	1152	Protein_79	trypsin-like peptidase domain-containing protein	-
HV139_RS24020	64914..65876	-	963	WP_021567591.1	M48 family metalloprotease	-
HV139_RS24025	65863..66351	-	489	WP_032731988.1	phosphate-starvation-inducible PsiE family protein	-
HV139_RS24030	66363..66605	-	243	WP_001446206.1	diguanylate cyclase	-
HV139_RS24035	66712..67428	-	717	Protein_83	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..270007	270007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13993.97 Da
• Isoelectric Point: 6.8515

>T163819 WP_025799003.1 NZ_CP056645:c62480-62103 [Citrobacter freundii]
MQTQPVPPTQEASPRPSPVEIWLRLLSHLLDRHYGLTLNDTPFCNDGVIQEHIDAGISLCDAVNFIVEKYALIRTDRHGF
SAETQSPLISSIDILRARKACGLMARNGYRLVTDITTGKYSEVTR

Nucleotide

• Length: 378 bp

>T163819 NZ_CP072763:2873151-2873564 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12016.71 Da
• Isoelectric Point: 6.4674

>AT163819 WP_072269511.1 NZ_CP056645:c62856-62530 [Citrobacter freundii]
IAEPWWGLKRDITPCFGARLVQEGNRLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLTSGELNPRHQHGVTLYA
KGLTCNADTLGSCGYVYIAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT163819 NZ_CP072763:2873603-2873860 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A097QYR4

Antitoxin

• AlphaFold DB: A0A7T0E0C6