Detailed information of TA system TA163815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4262626..4263202
• Replicon: chromosome
• Accession: NZ_CP056644
• Organism: Citrobacter freundii strain RHBSTW-00267

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV139_RS20510
• Protein ID: WP_117342369.1
• Coordinates: 4262915..4263202 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV139_RS20505
• Protein ID: WP_117342368.1
• Coordinates: 4262626..4262928 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV139_RS20490	4259094..4261244	+	2151	WP_096758430.1	pyruvate/proton symporter BtsT	-
HV139_RS20495	4261352..4261546	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HV139_RS20500	4261559..4262515	+	957	WP_096758431.1	GTPase	-
HV139_RS20505	4262626..4262928	-	303	WP_117342368.1	BrnA antitoxin family protein	Antitoxin
HV139_RS20510	4262915..4263202	-	288	WP_117342369.1	BrnT family toxin	Toxin
HV139_RS20515	4263375..4263539	-	165	WP_117342370.1	DUF1127 domain-containing protein	-
HV139_RS20520	4263716..4265128	+	1413	WP_146717581.1	PLP-dependent aminotransferase family protein	-
HV139_RS20525	4265155..4266105	-	951	WP_181629582.1	Rpn family recombination-promoting nuclease/putative transposase	-
HV139_RS20530	4266380..4267621	+	1242	WP_181218809.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11362.75 Da
• Isoelectric Point: 7.5239

>T163815 WP_117342369.1 NZ_CP056644:c4263202-4262915 [Citrobacter freundii]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHD

Nucleotide

• Length: 288 bp

>T163815 NZ_CP072763:c2367451-2367017 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11444.95 Da
• Isoelectric Point: 8.5366

>AT163815 WP_117342368.1 NZ_CP056644:c4262928-4262626 [Citrobacter freundii]
MSMIKHKRGSASALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGTFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT163815 NZ_CP072763:c2367712-2367458 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG