Detailed information of TA system TA163814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3735615..3736235
• Replicon: chromosome
• Accession: NZ_CP056644
• Organism: Citrobacter freundii strain RHBSTW-00267

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV139_RS17980
• Protein ID: WP_002892050.1
• Coordinates: 3736017..3736235 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV139_RS17975
• Protein ID: WP_115259201.1
• Coordinates: 3735615..3735989 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV139_RS17965	3730760..3731953	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV139_RS17970	3731976..3735125	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HV139_RS17975	3735615..3735989	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HV139_RS17980	3736017..3736235	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV139_RS17985	3736617..3737090	+	474	WP_096758041.1	YlaC family protein	-
HV139_RS17990	3737164..3737304	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV139_RS17995	3737306..3737566	-	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HV139_RS18000	3737751..3739304	+	1554	WP_181629219.1	EAL domain-containing protein	-
HV139_RS18005	3739358..3739711	-	354	WP_115259217.1	DUF1428 family protein	-
HV139_RS18010	3739794..3740405	-	612	WP_181629222.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T163814 WP_002892050.1 NZ_CP056644:3736017-3736235 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T163814 NZ_CP072763:2323988-2324398 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT163814 WP_115259201.1 NZ_CP056644:3735615-3735989 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT163814 NZ_CP072763:2323762-2323995 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2