Detailed information of TA system TA163800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 77018..77543
• Replicon: plasmid pRHBSTW-00310_3
• Accession: NZ_CP056624
• Organism: Citrobacter freundii strain RHBSTW-00310

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: HV151_RS26490
• Protein ID: WP_004197633.1
• Coordinates: 77238..77543 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HV151_RS26485
• Protein ID: WP_004197642.1
• Coordinates: 77018..77236 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV151_RS26445	72409..72612	-	204	WP_004197809.1	hypothetical protein	-
HV151_RS26450	72626..72829	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
HV151_RS26455	72863..73231	-	369	WP_004197807.1	hypothetical protein	-
HV151_RS26460	73275..73769	-	495	WP_020805591.1	hypothetical protein	-
HV151_RS26465	73800..74372	-	573	WP_020805590.1	hypothetical protein	-
HV151_RS26470	74369..74617	-	249	WP_000272716.1	hypothetical protein	-
HV151_RS26475	75054..75743	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
HV151_RS26480	75775..76464	-	690	WP_004193995.1	hypothetical protein	-
HV151_RS26485	77018..77236	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV151_RS26490	77238..77543	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV151_RS26495	77712..78113	+	402	WP_165590456.1	hypothetical protein	-
HV151_RS26500	78140..78463	+	324	WP_004197641.1	hypothetical protein	-
HV151_RS26505	78460..79476	+	1017	WP_004197639.1	hypothetical protein	-
HV151_RS26510	79674..80468	+	795	WP_004197635.1	site-specific integrase	-
HV151_RS26515	80932..81111	-	180	WP_006788217.1	hypothetical protein	-
HV151_RS26520	81231..81857	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152768	152768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T163800 WP_004197633.1 NZ_CP056624:77238-77543 [Citrobacter freundii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163800 NZ_CP072763:c1388984-1388691 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT163800 WP_004197642.1 NZ_CP056624:77018-77236 [Citrobacter freundii]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163800 NZ_CP072763:c1389250-1388981 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5